|
KMID : 1023420100100040227
|
|
Journal of Dental Hygiene Science
2010 Volume.10 No. 4 p.227 ~ p.231
|
|
|
MAPK Signal Pathways in Regulation of Odontoblastic Differentiation by Induction of HO-1 in Human Dental Pulp Cells
|
|
Kim Sun-Ju
|
|
|
Abstract
|
|
|
|
The purpose of this study was to examine the MAPK signaling pathways involved in regulation of HO-1 and the odontoblast differentiation markers during the odontoblastic differentiation for HDPCs. We evaluated cell growth by MTT assay and differentiation marker mRNA expression by RT-PCR. When the cells were treated with p38 inhibitor (SB203580, 10 μM), JNK inhibitor (SP600125, 10 μM), and ERK inhibitor (PD98059, 20 μM) for 7 days, cell growth and expression of HO-1 and differentiation makers were significantly decreased in HDPCs. Our results suggest that odontoblastic differentiation is positively regulated by HO-1 induction in HDPCs via ERK, JNK, and p38 signaling pathways. Thus, pharmacological HO-1 induction might represent a potent therapeutic approach for pulp capping and the regeneration of HDPCs.
|
|
|
KEYWORD
|
|
|
Human dental pulp cells, Mitogen-activated protein kinase, Heme oxygenase-1, Differentiation
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|
|